Mentors At Your Benchside

#8 — You probably have or will use SDS-PAGE at some point to separate proteins, but do you really understand how this technique works? Knowing how SDS-PAGE works means you can tweak and troubleshoot your technique as well as impress your supervisor and lab mates. In this episode, we take you through how SDS-PAGE works, including what SDS does, why you need to use a reducing agent like DTT or beta-mercaptoethanol, and the critical importance of the stacking gel. Read the full How SDS-PAGE Works article to see helpful visuals for how this technique works and access the table showing the protein sizes that different acrylamide percentages can separate. [1] Expand your knowledge by buffing up on Laemmli buffer and get our Guide to Gradient Gels. [2,3] If you pour your own SDS-PAGE gels, take a deep dive into the chemistry of how gels work and learn how to pour perfect gels every time with our Simple SDS-PAGE Gel Recipe with 10-Step Casting Protocol. [4] Resources: 1. How SDS-PAGE Works. Available at: https://